TRIM28 Antibody - Biotin Conjugated (OACA01202)

Catalog No: OACA01202

Size:100ug

All lanes : Transcription intermediary factor 1-beta antibody at at 2 ug/ml
Lane 1 :EC109 whole cell lysate
Lane 2 :293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 92 kDa
Observed band size: 92 kDa
 

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Biotin
• Application: Enzyme-linked immunosorbent assay
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Transcription intermediary factor 1-beta protein (22-291AA)
• Purification: Protein G Purified
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Reference: [1] KAP-1, a novel corepressor for the highly conserved KRAB repression domain.'Friedman J.R., Fredericks W.J., Jensen D.E., Speicher D.W., Huang X.-P., Neilson E.G., Rauscher F.J. IIIGenes Dev. 10:2067-2078(1996)
  [2] Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1.'Moosmann P.R., Georgiev O., le Douarin B., Bourquin J.-P., Schaffner W.Nucleic Acids Res. 24:4859-4867(1996)
  [3] The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).'The MGC Project TeamGenome Res. 14:2121-2127(2004)
• Storage Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Target Info

• Gene Symbol: TRIM28
• Gene Full Name: tripartite motif containing 28
• Alias Symbols: E3 SUMO-protein ligase TRIM28;KAP1;KAP-1;KRAB [Kruppel-associated box domain]-associated protein 1;KRAB-associated protein 1;KRAB-interacting protein 1;KRIP-1;nuclear corepressor KAP-1;PPP1R157;protein phosphatase 1, regulatory subunit 157;RING finger protein 96;RING-type E3 ubiquitin transferase TIF1-beta;RNF96;TF1B;TIF1B;TIF1-beta;transcription intermediary factor 1-beta;transcriptional intermediary factor 1-beta;Tripartite motif-containing protein 28.
• NCBI Gene Id: 10155
• Protein Name: Transcription intermediary factor 1-beta
• Description of Target: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity).
• Uniprot ID: Q13263