Catalog No: OKEH02533
Size:96T
Price: $400.00
SKU
OKEH02533
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Triiodothyronine ELISA Kit (OKEH02533) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Triiodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Triiodothyronine and incubated. The Triiodothyronine found in the sample or standards competes with the biotinylated Triiodothyronine for limited binding sites on the immobilized anti-Triiodothyronine antibody. Excess unbound biotinylated Triiodothyronine and sample or standard Triiodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Triiodothyronine captured in the well and inversely proportional to the amount of Triiodothyronine which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.2% (n = 20)
Mean Inter-assay CV%: < 10.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Triiodothyronine Microplate96 Wells (12 x 8 Well strips)
Triiodothyronine Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Triiodothyronine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: T3
Target Name: Triiodothyronine
::Pubchem: 44123465
::Chemical formula: C15H12I3NO4
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity35 pg/mL
SpecificityNatural and recombinant General Triiodothyronine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias Symbolstriiodothyronine, T3, 3,3',5-triiodo-L-thyronine
Protein NameTriiodothyronine
Description of TargetThe L-triiodothyronine (T3, liothyronine) thyroid hormone is normally synthesized and secreted by the thyroid gland in much smaller quantities than L-tetraiodothyronine (T4, levothyroxine, L-thyroxine). Most T3 is derived from peripheral monodeiodination of T4 at the 5 position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.
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