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Catalog No: OKEH03230
Size:96T
Price: $525.00
SKU
OKEH03230
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TRH ELISA Kit (Rat) (OKEH03230) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Trh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Trh and incubated. The Trh found in the sample or standards competes with the biotinylated Trh for limited binding sites on the immobilized anti-Rat Trh antibody. Excess unbound biotinylated Trh and sample or standard Trh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Trh captured in the well and inversely proportional to the amount of Trh which was contained in the sample or standard.
ELISA Kit Range0.156-10uIU/mL
ELISA Kit Component
ComponentAmount
Anti-Trh Microplate96 Wells (12 x 8 Well strips)
Trh Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Trh-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078uIU/mL
SpecificityNatural and recombinant Rat Pro-thyrotropin-releasing hormone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTrh
Gene Full Namethyrotropin releasing hormone
Alias Symbolsprothyroliberin;pro-thyrotropin-releasing hormone;protirelin;Pro-TRH;THR;thyrotropin releasing hormone;thyrotropin-releasing factor;Trf;TRH01;TSH-releasing factor.
NCBI Gene Id25569
Protein NameP01150
Description of TargetPro-thyrotropin-releasing hormone
Uniprot IDhttps://www.uniprot.org/uniprot/P01150
Protein Accession #NP_037178.1
Nucleotide Accession #NM_013046.3
Protein Size (# AA)255
Molecular Weight29 kDa
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