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Catalog No: OKCD01369
Size:96WELLS
Price: $450.00
SKU
OKCD01369
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Trf High Sensitivity ELISA Kit (Mouse) (OKCD01369)

Datasheets/ManualsPrintable datasheet for Trf High Sensitivity ELISA Kit (Mouse) (OKCD01369)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-103%90-97%90-101%81-89%
EDTA Plasma (n=5)94-103%80-92%93-101%87-101%
Heparin Plasma (n=5)97-104%84-98%86-102%78-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Transferrin (TF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Transferrin (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Transferrin (TF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Transferrin (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-10197
EDTA Plasma (n=5)85-9994
Heparin Plasma (n=5)81-10589
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Transferrin (TF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Transferrin (TF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Trf Microplate96 Wells (12 x 8 Well Strips)
Trf Lyophilized Standard2
100X Biotinylated Trf Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity0.051 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTrf
Gene Full Nametransferrin
Alias SymbolsAI266983;beta-1 metal-binding globulin;Cd176;H;HP;hpx;hypotransferrinemia with hemochromatosis;serotransferrin;siderophilin;Tf;Tfn.
NCBI Gene Id22041
Protein NameSerotransferrin
Description of TargetTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Uniprot IDQ921I1
Protein Accession #NP_598738.1
Nucleotide Accession #NM_133977.2
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