Catalog No: OKEH00303
Size:96T
Price: $425.00
SKU
OKEH00303
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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TREM1 ELISA Kit (Human) : 96 Wells (OKEH00303)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00303
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TREM1 ELISA Kit (Human) (OKEH00303) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TREM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for TREM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TREM1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Recovery<= 4.1%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 10.8% (n = 20)
ELISA Kit Component
ComponentAmount
TREM1 Microplate96 Wells (12 x 8 Well strips)
TREM1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TREM1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity29.13 pg/mL
SpecificityNatural and recombinant Human Triggering receptor expressed on myeloid cells 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTREM1
Gene Full Nametriggering receptor expressed on myeloid cells 1
Alias SymbolsCD354;TREM-1;triggering receptor expressed on monocytes 1;triggering receptor expressed on myeloid cells 1.
NCBI Gene Id54210
Protein NameQ9NP99
Description of TargetTriggering receptor expressed on myeloid cells 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NP99
Protein Accession #NP_001229518.1
Nucleotide Accession #NM_001242589.2
Protein Size (# AA)234
Molecular Weight26 kDa
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