TREM-1 ELISA Kit (Mouse) (OKBB00451)

Catalog No: OKBB00451

Size:96 Wells

Mouse TREM-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TREM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TREM-1 has been precoated onto 96-well plates. Standards(NS0, A21-S202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TREM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TREM-1 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	252	559	924	314	675	1104
  SD	14.93	39.13	42.5	24.5	48.6	60.72
  CV%	5.9	7	4.6	7.8	7.2	5.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TREM-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TREM-1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TREM-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA), Cell lysates and tissue homogenates
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TREM-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A21-S202
• Description: For quantitative detection of mouse TREM-1 in cell culture supernatants, serum, plasma(heparin, EDTA) and cell lysates.

Target Info

• Gene Symbol: TREM1
• Gene Full Name: triggering receptor expressed on myeloid cells 1
• Alias Symbols: CD354, TREM 1Triggering receptor expressed on monocytes 1, Triggering receptor expressed on myeloid cells 1, Triggering receptor TREM 1, Triggering receptor TREM1
• NCBI Gene Id: 58217
• Protein Name: Triggering receptor expressed on myeloid cells 1
• Description of Target: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
• Uniprot ID: Q9JKE2
• Protein Accession #: NP_067381.1
• Nucleotide Accession #: NM_021406.5