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Catalog No: OKAG00259 (Formerly GWB-SKR259)
Size:1 plate: 12 x 8-Well Microstrips
Price: $475.00
SKU
OKAG00259
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleThe Aviva Human TREM-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hTREM-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human TREM-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range94-6,000 pg/mL
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (18 ng)
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, sera and plasma
Sensitivity94 pg/mL
SpecificityThe Human TREM-1 ELISA is capable of recognizing both recombinant and naturally produced Human TREM-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Murine: TREM-1, TREM-2b
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolTREM1
Gene Full Nametriggering receptor expressed on myeloid cells 1
Alias SymbolsCD354, TREM-1
NCBI Gene Id54210
Protein NameTriggering receptor expressed on myeloid cells 1
Description of TargetThis gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Uniprot IDQ9NP99
Protein Accession #NP_001229518.1
Nucleotide Accession #NM_001242589.1
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