TRA2B ELISA Kit (Human) (OKCD00619)

Catalog No: OKCD00619 (Formerly GWB-KBBYU4)

Size:96WELLS

Human TRA2B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta (TRA2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transformer 2 Beta (TRA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta (TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transformer 2 Beta (TRA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TRA2B Microplate: 96 Wells (12 x 8 Well Strips)
  • TRA2B Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TRA2B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transformer 2 Beta (TRA2b). No significant cross-reactivity or interference between Transformer 2 Beta (TRA2b) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TRA2B
• Gene Full Name: transformer 2 beta homolog
• Alias Symbols: Htra2-beta;PPP1R156;protein phosphatase 1, regulatory subunit 156;SFRS10;Splicing factor, arginine/serine-rich 10;splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila);SRFS10;TRA-2 beta;TRA2-BETA;TRAN2B;transformer-2 protein homolog B;transformer-2 protein homolog beta.
• NCBI Gene Id: 6434
• Protein Name: Transformer-2 protein homolog beta
• Description of Target: Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.
• Uniprot ID: P62995
• Protein Accession #: NP_001230808
• Nucleotide Accession #: NM_001243879.1
• Protein Size (# AA): 288