Catalog No: OKEH03074
Size:96T
Price: $600.00
SKU
OKEH03074
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TPSB2 ELISA Kit (Mouse) (OKEH03074) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tpsb2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tpsb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tpsb2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.9% (n = 20)
Mean Inter-assay CV%: <= 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Tpsb2 Microplate96 Wells (12 x 8 Well strips)
Tpsb2 Lyophilized Standard2
100X Biotinylated Tpsb2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.081 ng/mL
SpecificityNatural and recombinant Mouse Tryptase beta-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTPSB2
Gene Full Nametryptase beta 2
Alias SymbolsMast cell protease 6.
NCBI Gene Id17229
Protein NameP21845
Description of TargetTryptase beta-2
Uniprot IDhttps://www.uniprot.org/uniprot/P21845
Protein Accession #NP_034911.3 
Nucleotide Accession #NM_010781.3
Protein Size (# AA)276
Molecular Weight31 kDa
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