TPSB2 ELISA Kit (Human) (OKCD00408)

Catalog No: OKCD00408 (Formerly GWB-KBBKU2)

Size:96WELLS

Human TPSB2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	94-101%	80-99%	94-102%	99-105%
  EDTA plasma(n=5)	81-94%	78-93%	94-105%	97-105%
  heparin plasma(n=5)	93-105%	98-105%	78-91%	91-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase Beta 2 (TPSb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptase Beta 2 (TPSb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptase Beta 2 (TPSb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tryptase Beta 2 (TPSb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	95-104	99
  EDTA plasma(n=5)	90-98	93
  heparin plasma(n=5)	78-89	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase Beta 2 (TPSb2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase Beta 2 (TPSb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TPSB2 Microplate: 96 Wells (12 x 8 Well Strips)
  • TPSB2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TPSB2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptase Beta 2 (TPSb2). No significant cross-reactivity or interference between Tryptase Beta 2 (TPSb2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TPSAB1|TPSB2
• Gene Full Name: tryptase alpha/beta 1|tryptase beta 2
• Alias Symbols: epididymis secretory sperm binding protein;mast cell alpha II tryptase;mast cell beta I tryptase;mast cell tryptase beta II;mast cell tryptase beta III;testicular tissue protein Li 163;TPS1;TPS2;TPSB1;TPSB2;tryptase alpha II;tryptase alpha/beta-1;tryptase alpha-1;tryptase beta I;tryptase beta-1;Tryptase beta-2;Tryptase II;tryptase-1;Tryptase-2;tryptaseB;tryptaseC;tryptase-I;tryptase-III.
• NCBI Gene Id: 64499|7177
• Protein Name: Tryptase beta-2
• Description of Target: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
• Uniprot ID: P20231
• Protein Accession #: NP_003285
• Nucleotide Accession #: NM_003294.3
• Protein Size (# AA): 275