Catalog No: OKEH04556
Size:96T
Price: $525.00
SKU
OKEH04556
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology TPO ELISA Kit (Human) (OKEH04556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TPO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TPO captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-TPO Microplate96 Wells (12 x 8 Well Strips)
TPO Lyophilized Standard2
Sample Diluent1 x 20 mL
TPO Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Human Thyroid peroxidase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTPO
Gene Full Namethyroid peroxidase
Alias SymbolsMSA;TDH2A;thyroid microsomal antigen;thyroid peroxidase;thyroperoxidase;TPX.
NCBI Gene Id7173
Protein NameP07202
Description of TargetThyroid peroxidase
Uniprot IDhttps://www.uniprot.org/uniprot/P07202
Protein Accession #NP_000538.3
Nucleotide Accession #NM_000547.5
Protein Size (# AA)993
Molecular Weight103 KDa
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