Catalog No: OKCD00743 (Formerly GWB-KBBVF7)
Size:96WELLS
Price: $550.00
SKU
OKCD00743
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for TNNI3 ELISA Kit (Human) (OKCD00743)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-97%79-93%95-102%87-104%
EDTA plasma(n=5)83-92%89-101%84-102%80-105%
heparin plasma(n=5)80-90%91-101%82-97%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cardiac Troponin I (cTnI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cardiac Troponin I (cTnI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cardiac Troponin I (cTnI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cardiac Troponin I (cTnI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)98-105102
EDTA plasma(n=5)91-9994
heparin plasma(n=5)78-10289
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TNNI3 Microplate96 Wells (12 x 8 Well Strips)
TNNI3 Lyophilized Standard2 x 2 ng
100X Biotinylated TNNI3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 13.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Troponin I Type 3, Cardiac (TNNI3). No significant cross-reactivity or interference between Troponin I Type 3, Cardiac (TNNI3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTNNI3
Gene Full Nametroponin I3, cardiac type
Alias SymbolsCardiac troponin I;cardiomyopathy, dilated 2A (autosomal recessive);CMD1FF;CMD2A;CMH7;cTnI;RCM1;TNNC1;troponin I type 3 (cardiac);troponin I, cardiac muscle.
NCBI Gene Id7137
Protein NameTroponin I, cardiac muscle
Description of TargetTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Uniprot IDP19429
Protein Accession #NP_000354
Nucleotide Accession #NM_000363.4
Protein Size (# AA)210
Write Your Own Review
You're reviewing:TNNI3 ELISA Kit (Human) (OKCD00743)
Your Rating
We found other products you might like!