TNFSF14 ELISA Kit (Mouse) (OKCD02944)

Catalog No: OKCD02944

Size:96WELLS

Mouse TNFSF14 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-96%	78-105%	96-103%	82-99%
  EDTA Plasma (n=5)	84-102%	97-105%	96-103%	80-104%
  Heparin Plasma (n=5)	88-95%	94-102%	83-101%	90-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-88	83
  EDTA Plasma (n=5)	92-101	96
  Heparin Plasma(n=5)	87-95	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TNFSF14 Microplate: 96 Wells (12 x 8 Well strips)
  • TNFSF14 Lyophilized Standard: 2
  • 100X Biotinylated TNFSF14 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 6.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tnfsf14
• Gene Full Name: tumor necrosis factor (ligand) superfamily, member 14
• Alias Symbols: HVEM;HVEML;HVEM-L;LIG;LIGHT;LTg;Ly113;Tnlg1d;tumor necrosis factor ligand 1d;tumor necrosis factor ligand superfamily member 14.
• NCBI Gene Id: 50930
• Protein Name: Tumor necrosis factor ligand superfamily member 14
• Description of Target: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB and stimulates the proliferation of T-cells.
• Uniprot ID: Q9QYH9
• Protein Accession #: NP_062291.1
• Nucleotide Accession #: NM_019418.3
• Protein Size (# AA): 239