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Catalog No: OKEH02943
Size:96T
Price: $425.00
SKU
OKEH02943
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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TNFSF14 ELISA Kit (Human) : 96 Wells (OKEH02943)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TNFSF14 ELISA Kit (Human) (OKEH02943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF14 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.3% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
TNFSF14 Microplate96 Wells (12 x 8 Well strips)
TNFSF14 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TNFSF14 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Tumor necrosis factor ligand superfamily member 14
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTNFSF14
Gene Full NameTNF superfamily member 14
Alias SymbolsCD258;Herpes virus entry mediator ligand;herpesvirus entry mediator ligand;HVEML;LIGHT;LTg;tumor necrosis factor (ligand) superfamily, member 14;tumor necrosis factor ligand 1D;tumor necrosis factor ligand superfamily member 14;tumor necrosis factor superfamily member 14.
NCBI Gene Id8740
Protein NameO43557
Description of TargetTumor necrosis factor ligand superfamily member 14
Uniprot IDhttps://www.uniprot.org/uniprot/O43557
Protein Accession #NP_003798.2
Nucleotide Accession #NM_003807.4
Protein Size (# AA)240
Molecular Weight26 kDa
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