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Catalog No: OKBB00378
Size:96 Wells
Price: $583.00
SKU
OKBB00378
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NS0,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TNFSF13 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)6532945563386432145366
SD26.8156.1219.742.4202.5268.3
CV%4.15.33.94.96.35
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human TNFSF13 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human TNFSF13 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human TNFSF13 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human TNFSF13
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: A105-L250
DescriptionFor quantitative detection of human TNFSF13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTNFSF13
Gene Full Nametumor necrosis factor (ligand) superfamily, member 13
Alias SymbolsA proliferation-inducing ligand, APRIL, CD256, TALL-2, TALL2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TNF13_HUMAN, TNFSF13, TRDL-1, Tumor necrosis factor (ligand) superfamily member 13, Tumor necrosis factor ligand superfamily member 13, Tumor necrosis factor like protein ZTNF2, Tumor necrosis factor related death ligand 1, ZTNF2
NCBI Gene Id8741
Protein NameTumor necrosis factor ligand superfamily member 13
Description of TargetTumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.
Uniprot IDO75888
Protein Accession #NP_001185551.1
Nucleotide Accession #NM_001198622.1
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