Catalog No: OKBB00413
Size:96 Wells
Price: $583.00
SKU
OKBB00413
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human TNFRSF17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF17 has been precoated onto 96-well plates. Standards(NS0, M1-A54) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TNFRSF17 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)13464212861586141358
SD4.6935.9561.736.6441.1473.33
CV%3.55.64.84.26.75.4
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human TNFRSF17 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human TNFRSF17 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human TNFRSF17 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human TNFRSF17
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: M1-A54
DescriptionFor quantitative detection of human TNFRSF17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTNFRSF17
Gene Full Nametumor necrosis factor receptor superfamily, member 17
Alias SymbolsB cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, BCMA, CD 269, CD269, CD269 antigen, TNFRSF 17, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor member 17, Tumor necrosis factor receptor superfamily member 17
NCBI Gene Id608
Protein NameTumor necrosis factor receptor superfamily member 17
Description of TargetTNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
Uniprot IDQ02223
Protein Accession #NP_001183.2
Nucleotide Accession #NM_001192.2
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