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Catalog No: OKBB00500
Size:96 Wells
Price: $583.00
SKU
OKBB00500
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NS0, S10-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TNFRSF13C amount of sample captured in plate.
ELISA Kit Range78 pg/ml - 5,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)5621250284163816343205
SD25.342.514235.776.8205.1
CV%4.53.455.64.76.4
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse TNFRSF13C Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse TNFRSF13C Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse TNFRSF13C Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse TNFRSF13C
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: S10-A71
DescriptionFor quantitative detection of mouse TNFRSF13C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTNFRSF13C
Gene Full Nametumor necrosis factor receptor superfamily, member 13C
Alias SymbolsB Cell Activating Factor Receptor, B-cell-activating factor receptor, BAFF R, BAFF receptor, BAFF-R, BAFFR, BLyS receptor 3, BlySR3, BR 3, BR3, BROMIX, CD 268, CD268, CD268 antigen, CVID4, MGC138235, OTTHUMP00000028746, Prolixin, TNFRSF 13C, TNFRSF13C, TR13C_HUMAN, Tumor Necrosis Factor Receptor Subunit Member 13C, Tumor necrosis factor receptor superfamily member 13C
NCBI Gene Id72049
Protein NameTumor necrosis factor receptor superfamily member 13C
Description of TargetTumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Uniprot IDQ9D8D0
Protein Accession #NP_082351.1
Nucleotide Accession #NM_028075.2
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