Catalog No: OKBB00514
Size:96 Wells
Price: $583.00
SKU
OKBB00514
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NS0, A2-C128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TNFRSF13B amount of sample captured in plate.
ELISA Kit Range46.9 pg/ml - 3,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)367926162424110681867
SD16.535.286.113.550.2113.9
CV%4.53.85.35.64.76.1
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse TACI Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse TACI Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse TACI Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse TNFRSF13B
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: A2-C128
DescriptionFor quantitative detection of mouse TNFRSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTNFRSF13B
Gene Full Nametumor necrosis factor receptor superfamily, member 13B
Alias SymbolsCD267, TNFRSF13B, TR13B_HUMAN, Transmembrane activator and CAML interactor, Tumor necrosis factor receptor superfamily member 13B
NCBI Gene Id57916
Protein NameTumor necrosis factor receptor superfamily member 13B
Description of TargetTumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
Uniprot IDQ9ET35
Protein Accession #NP_067324.1
Nucleotide Accession #NM_021349.1
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