TMEM173 ELISA Kit (Human) (OKCD00623)

Catalog No: OKCD00623 (Formerly GWB-KBBOB4)

Size:96WELLS

Human TMEM173 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-101%	78-102%	80-95%	85-92%
  EDTA Plasma (n=5)	89-97%	79-89%	80-102%	78-105%
  Heparin Plasma (n=5)	82-102%	95-102%	96-104%	84-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 173 (TMEM173). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 173 (TMEM173). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 173 (TMEM173), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protein 173 (TMEM173) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	81-103	90
  EDTA plasma(n=5)	78-89	82
  heparin plasma(n=5)	83-92	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transmembrane Protein 173 (TMEM173) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transmembrane Protein 173 (TMEM173) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TMEM173 Microplate: 96 Wells (12 x 8 Well Strips)
  • TMEM173 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TMEM173 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 173 (TMEM173). No significant cross-reactivity or interference between Transmembrane Protein 173 (TMEM173) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: STING1
• Gene Full Name: stimulator of interferon response cGAMP interactor 1
• Alias Symbols: endoplasmic reticulum IFN stimulator;endoplasmic reticulum interferon stimulator;ERIS;hMITA;hSTING;Mediator of IRF3 activation;MITA;mitochondrial mediator of IRF3 activation;MPYS;NET23;N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner;SAVI;stimulator of interferon genes protein;stimulator of interferon protein;STING;STING-beta;TMEM173;transmembrane protein 173.
• NCBI Gene Id: 340061
• Protein Name: Stimulator of interferon genes protein
• Description of Target: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Essential for the induction of IFN-beta in response to human herpes simplex virus 1 (HHV-1) infection. Exhibits 2',3' phosphodiester linkage-specific ligand recognition. Can bind both 2'-3' linked cGAMP and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed:26300263).
• Uniprot ID: Q86WV6
• Protein Accession #: NP_938023
• Nucleotide Accession #: NM_198282.3
• Protein Size (# AA): 379