TMEM119 ELISA Kit (Human) (OKCA01559)

Catalog No: OKCA01559

Size:96 Wells

Human TMEM119 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	107	103-110
  1:2	88	84-92
  1:4	107	103-110
  1:8	90	87-93

• ELISA Kit Principle: Aviva Systems Biology TMEM119 ELISA Kit (Human) (OKCA01559) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMEM119 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM119 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TMEM119 captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	104	100-108
  EDTA Plasma (n=4)	88	84-93

• ELISA Kit Component:
  • TMEM119 Microplate: 96 Wells (12 x 8 Well strips)
  • TMEM119 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated TMEM119 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: TMEM119
• Gene Full Name: transmembrane protein 119
• Alias Symbols: OBIF
• NCBI Gene Id: 338773
• Protein Name: Transmembrane protein 119
• Description of Target: Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
• Uniprot ID: Q4V9L6
• Protein Accession #: NP_859075.2
• Nucleotide Accession #: NM_181724.2