TLR8 ELISA Kit (Human) (OKCD02935)

Catalog No: OKCD02935

Size:96WELLS

Human TLR8 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-105%	78-94%	89-97%	80-91%
  EDTA Plasma (n=5)	84-103%	84-105%	98-105%	98-105%
  Heparin Plasma (n=5)	84-91%	92-101%	89-104%	90-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 8 (TLR8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 8 (TLR8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 8 (TLR8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Toll Like Receptor 8 (TLR8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-103	97
  EDTA Plasma (n=5)	82-97	88
  Heparin Plasma(n=5)	88-95	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 8 (TLR8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 8 (TLR8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TLR8 Microplate: 96 Wells (12 x 8 Well strips)
  • TLR8 Lyophilized Standard: 2
  • 100X Biotinylated TLR8 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.108 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 8 (TLR8). No significant cross-reactivity or interference between Toll Like Receptor 8 (TLR8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TLR8
• Gene Full Name: toll like receptor 8
• Alias Symbols: CD288;hTLR8;toll-like receptor 8.
• NCBI Gene Id: 51311
• Protein Name: Toll-like receptor 8
• Description of Target: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
• Uniprot ID: Q9NR97
• Protein Accession #: NP_057694.2
• Nucleotide Accession #: NM_016610.3
• Protein Size (# AA): 1041