TLR6 Antibody (OAPB00363)

Catalog No: OAPB00363

Size:100UG

Western blot analysis of TLR6 in Jurkat cell lysate with TLR6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

Immunocytochemistry of TLR6 in Jurkat cells with TLR6 antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. TLR6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: TLR6 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: TLR6 antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human TLR6. The immunogen is located within amino acids 160 - 210 of TLR6.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TLR6 antibody is Catalog # OEPB02466
• Application Info: TLR6 antibody can be used for detection of TLR6 by Western blot at 1 to 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.;Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.;McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm.2004; 41:577-82.;Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding Toll-like receptor family. Gene1999; 231:59-65.

Target Info

• Gene Symbol: TLR6
• Gene Full Name: toll like receptor 6
• Alias Symbols: CD286;toll-like receptor 6.
• NCBI Gene Id: 10333
• Protein Name: Toll-like receptor 6|toll-like receptor 6 precursor [Homo sapiens]
• Description of Target: Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:20037584). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2 (PubMed:11441107). In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:11441107, PubMed:20037584).
• Uniprot ID: Q9Y2C9
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_006059
• Molecular Weight: Observed: 91 kDa