TLR2 ELISA Kit (Mouse) (OKBB00506)

Catalog No: OKBB00506

Size:96 Wells

Mouse TLR2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TLR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TLR2 has been precoated onto 96-well plates. Standards(NS0, Q25-L590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TLR2 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.05	2.83	5.62	1.38	3.22	5.79
  SD	0.036	0.116	0.253	0.077	0.206	0.388
  CV%	3.4	4.1	4.5	5.6	6.4	6.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TLR2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TLR2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TLR2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TLR2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q25-L590
• Description: For quantitative detection of mouse TLR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: TLR2
• Gene Full Name: toll-like receptor 2
• Alias Symbols: CD_antigen=CD282, CD282, TIL4, Toll like receptor 2, Toll/interleukin 1 receptor like protein 4
• NCBI Gene Id: 24088
• Protein Name: Toll-like receptor 2
• Description of Target: Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
• Uniprot ID: Q9QUN7
• Protein Accession #: NP_036035.3
• Nucleotide Accession #: NM_011905.3