Catalog No: OKEH03030
Size:96T
Price: $525.00
SKU
OKEH03030
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TKT ELISA Kit (Human) (OKEH03030) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TKT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for TKT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TKT captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TKT Microplate96 Wells (12 x 8 Well strips)
TKT Lyophilized Standard2
100X Biotinylated TKT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Transketolase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTKT
Gene Full Nametransketolase
Alias Symbolsepididymis luminal protein 107;epididymis secretory protein Li 48;HEL107;HEL-S-48;SDDHD;TK;TKT1;transketolase.
NCBI Gene Id7086
Protein NameP29401
Description of TargetTransketolase
Uniprot IDhttps://www.uniprot.org/uniprot/P29401
Protein Accession #NP_001055.1
Nucleotide Accession #NM_001064.3
Protein Size (# AA)623
Molecular Weight68 kDa
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