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Catalog No: OKEH04778
Size:96T
Price: $525.00
SKU
OKEH04778
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Tk1 ELISA Kit (Rat) (OKEH04778) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tk1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Tk1 Microplate96 Wells (12 x 8 Well Strips)
Tk1 Standard2
Sample Diluent1 x 20 mL
Tk1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Thymidine kinase, cytosolic
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTk1
Gene Full Namethymidine kinase 1
Alias Symbolsthymidine kinase 1, soluble;thymidine kinase, cytosolic;Tk.
NCBI Gene Id24834
Protein NameP27158
Description of TargetThymidine kinase, cytosolic
Uniprot IDhttps://www.uniprot.org/uniprot/P27158
Protein Accession #NP_434687.1
Nucleotide Accession #NM_052800.1
Protein Size (# AA)121
Molecular Weight14 kDa
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