TJP1 ELISA Kit (Human) (OKDD00562)

Catalog No: OKDD00562 (Formerly GWB-KBBSF4)

Size:96 Wells

Human TJP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology TJP1 ELISA Kit (Human) (OKDD00562) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for TJP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TJP1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TJP1 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TJP1 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-TJP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TJP1 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X TJP1 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, cell lysates
• Sensitivity: < 0.068 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of TJP1. No significant cross-reactivity or interference between TJP1 and analogues was observed.
• Publications:

  Tight junction protein ZO-1 in Kawasaki disease. BMC Pediatr. 21, 157 (2021)

• Assay Info: Quantitative Sandwich ELISA
• Description:
  View product citations for TJP1 ELISA Kit (Human) (OKDD00562) on CiteAb

Target Info

• Gene Symbol: TJP1
• Gene Full Name: tight junction protein 1
• Alias Symbols: Tight junction protein 1;tight junction protein ZO-1;ZO-1;zona occludens 1;Zona occludens protein 1;zonula occludens 1 protein;Zonula occludens protein 1.
• NCBI Gene Id: 7082
• Protein Name: Tight junction protein ZO-1
• Description of Target: TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).
• Uniprot ID: Q07157
• Protein Accession #: NP_003248.3
• Nucleotide Accession #: NM_003257.4
• Protein Size (# AA): 1748
• Molecular Weight: 195 kDa