Catalog No: OKCA01104
Size:96 Wells
Price: $775.00
SKU
OKCA01104
Availability: Discontinued
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- Toll Free: 888-880-0001
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Tissue Transglutaminase Antibody (IgG) (Human) ELISA Kit (OKCA01104)
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Tissue Transglutaminase Antibody (IgG) (Human) ELISA Kit (OKCA01104) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Tissue Transglutaminase antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Tissue Transglutaminase Antibody (IgG) captured in well. | ||||||||||||||||||||
ELISA Kit Reproducibility | Three different samples of known Tissue Transglutaminase Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each. Intra-Assay CV: <15% Inter-Assay CV: <15% | ||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Serum | ||||||||||||||||||||
Assay Info | Assay Methodology: Qualitative Reverse Capture ELISA | ||||||||||||||||||||
Protocol Information |
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Alias Symbols | tTG |
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Protein Name | Tissue Transglutaminase Antibody (IgG) |
Description of Target | Tissue Transglutaminase Antibody (IgG) |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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