Catalog No: OKBB00607
Size:96 Wells
Price: $583.00
SKU
OKBB00607
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse TIMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-4 has been precoated onto 96-well plates. Standards(NS0, C30-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TIMP-4 amount of sample captured in plate.
ELISA Kit Range78 pg/ml - 5,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)27283019342967931615
SD12.24473.198.622.553.92116.3
CV%4.55.75.17.66.87.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse TIMP-4 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse TIMP-4 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse TIMP-4 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse TIMP-4
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: C30-P224
DescriptionFor quantitative detection of mouse TIMP-4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTIMP4
Gene Full NameTIMP metallopeptidase inhibitor 4
Alias SymbolsMetalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4
NCBI Gene Id110595
Protein NameMetalloproteinase inhibitor 4
Description of TargetMetalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
Uniprot IDQ9JHB3
Protein Accession #NP_542370.3
Nucleotide Accession #NM_080639.3
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