TIMP-2 ELISA Kit (Mouse) (OKBB00476)

Catalog No: OKBB00476

Size:96 Wells

Mouse TIMP-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-2 has been precoated onto 96-well plates. Standards(NS0, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TIMP-2 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.24	3.85	6.85	1.32	3.96	6.88
  SD	0.056	0.146	0.219	0.088	0.245	0.516
  CV%	4.5	3.8	3.2	6.7	6.2	7.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TIMP-2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TIMP-2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TIMP-2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TIMP-2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: C27-P220
• Description: For quantitative detection of mouse TIMP-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: TIMP2
• Gene Full Name: TIMP metallopeptidase inhibitor 2
• Alias Symbols: CSC 21K, CSC21K, D11Bwg1104e, DDC8, Metalloproteinase inhibitor 2, Metalloproteinase inhibitor 2 precursor, MGC105282, OTTMUSP00000004170, OTTMUSP00000004171, RP23-394O9.2, TIMP 2, TIMP metallopeptidase inhibitor 2, Tissue inhibitor of metalloproteinase 2, Tissue inhibitor of metalloproteinases 2
• NCBI Gene Id: 21858
• Protein Name: Metalloproteinase inhibitor 2
• Description of Target: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
• Uniprot ID: P25785
• Protein Accession #: NP_035724
• Nucleotide Accession #: NM_011594