TIMP-1 ELISA Kit (Mouse) (OKBB00403)

Catalog No: OKBB00403

Size:96 Wells

Mouse TIMP-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-1 has been precoated onto 96-well plates. Standards(NS0, C25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TIMP-1 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	371	602	1025	382	619	1270
  SD	15.95	29.5	36.9	24.45	47.04	73.66
  CV%	4.3	4.9	3.6	6.4	7.6	5.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse TIMP-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse TIMP-1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse TIMP-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse TIMP-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: C25-R205
• Description: For quantitative detection of mouse TIMP-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: TIMP1
• Gene Full Name: TIMP metallopeptidase inhibitor 1
• Alias Symbols: C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CCR5_HUMAN, CD195, CHEMR13, CLGI, Collagenase inhibitor, EPA, EPO, Erythroid potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, HIV-1 fusion coreceptor, Human Collagenase Inhibitor, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, OTTHUMP00000023214, TIMP, TIMP 1, TIMP metallopeptidase inhibitor 1, TIMP1 protein, Tissue Inhibitor of metalloproteinase 1, Tissue inhibitor of metalloproteinases, Tissue inhibitor of metalloproteinases 1, Tissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
• NCBI Gene Id: 21857
• Protein Name: Metalloproteinase inhibitor 1
• Description of Target: TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What’s more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.
• Uniprot ID: P12032
• Protein Accession #: NP_001037849.1
• Nucleotide Accession #: NM_001044384.1