THY1 ELISA Kit (Mouse) (OKCD02928)

Catalog No: OKCD02928

Size:96WELLS

Mouse THY1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-103%	83-92%	83-95%	86-101%
  EDTA Plasma (n=5)	84-95%	81-102%	84-94%	78-103%
  Heparin Plasma (n=5)	78-92%	88-96%	98-105%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 90 (CD90). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster of Differentiation 90 (CD90). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 90 (CD90), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster of Differentiation 90 (CD90) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.50-800pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-97	94
  EDTA Plasma (n=5)	91-105	95
  Heparin Plasma(n=5)	87-102	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 90 (CD90) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 90 (CD90) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-THY1 Microplate: 96 Wells (12 x 8 Well strips)
  • THY1 Lyophilized Standard: 2
  • 100X Biotinylated THY1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 5.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thy1 Cell Surface Antigen (Thy1). No significant cross-reactivity or interference between Thy1 Cell Surface Antigen (Thy1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Thy1
• Gene Full Name: thymus cell antigen 1, theta
• Alias Symbols: CD90;T25;Thy-1;thy-1 antigen;thy-1 membrane glycoprotein;Thy1.1;Thy1.2;Thy-1.2.
• NCBI Gene Id: 21838
• Protein Name: Thy-1 membrane glycoprotein
• Description of Target: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
• Uniprot ID: P01831
• Protein Accession #: NP_033408.1
• Nucleotide Accession #: NM_009382.3
• Protein Size (# AA): 162