THRSP ELISA Kit (Human) (OKCD00351)

Catalog No: OKCD00351 (Formerly GWB-KBBCO8)

Size:96WELLS

Human THRSP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-96%	80-91%	86-94%	92-101%
  EDTA plasma(n=5)	97-105%	80-99%	80-99%	80-95%
  heparin plasma(n=5)	90-99%	88-101%	88-96%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Responsive (THRSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Responsive (THRSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Responsive (THRSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyroid Hormone Responsive (THRSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-98	94
  EDTA plasma(n=5)	80-88	84
  heparin plasma(n=5)	78-93	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-THRSP Microplate: 96 Wells (12 x 8 Well Strips)
  • THRSP Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated THRSP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Responsive (THRSP). No significant cross-reactivity or interference between Thyroid Hormone Responsive (THRSP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: THRSP
• Gene Full Name: thyroid hormone responsive
• Alias Symbols: lipogenic protein 1;Lpgp;LPGP1;S14;spot 14 protein;SPOT14;SPOT14 homolog;THRP;thyroid hormone responsive (SPOT14 homolog, rat);thyroid hormone-inducible hepatic protein.
• NCBI Gene Id: 7069
• Protein Name: Thyroid hormone-inducible hepatic protein
• Description of Target: Plays a role in the regulation of lipogenesis, especially in lactating mammary gland. Important for the biosynthesis of triglycerides with medium-length fatty acid chains. May modulate lipogenesis by interacting with MID1IP1 and preventing its interaction with ACACA (By similarity). May function as transcriptional coactivator. May modulate the transcription factor activity of THRB.
• Uniprot ID: Q92748
• Protein Accession #: NP_003242
• Nucleotide Accession #: NM_003251.3
• Protein Size (# AA): 146