Thromboxane B2 ELISA Kit (OKEH02562)

Catalog No: OKEH02562

Size:96T

Thromboxane B2 ELISA Kit (OKEH02562) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Thromboxane B2 ELISA Kit (OKEH02562) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Thromboxane B2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thromboxane B2 and incubated. The Thromboxane B2 found in the sample or standards competes with the biotinylated Thromboxane B2 for limited binding sites on the immobilized anti-Thromboxane B2 antibody. Excess unbound biotinylated Thromboxane B2 and sample or standard Thromboxane B2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thromboxane B2 captured in the well and inversely proportional to the amount of Thromboxane B2 which was contained in the sample or standard.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.1% (n = 20)
  Mean Inter-assay CV%: < 10.2% (n = 20)
• ELISA Kit Component:
  • Anti-Thromboxane B2 Microplate: 96 Wells (12 x 8 Well strips)
  • Thromboxane B2 Lyophilized Standard: 2 x 1,000 pg
  • Sample Diluent: 1 x 20 mL
  • 100X Thromboxane B2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: TXB2
  Target Name: Thromboxane B2
  Pubchem: 5283137
  Chemical formula: C20H34O6
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 5.7 pg/mL
• Specificity: Natural and recombinant General Thromboxane B2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: TXB2, Thromboxane B2, TXB2
• Protein Name: Thromboxane B2
• Description of Target: Thromboxane B2 is an inactive metabolite/product of thromboxane A2. It is almost completely cleared in the urine. It itself is not involved in platelet activation and aggregation in case of a wound, but its precursor, thromboxane A2, is. Thromboxane A2 synthesis is the target of the drug aspirin, which inhibits the COX-1 enzyme (the source of thromboxane A2 in platelets).