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Catalog No: OKAG00254 (Formerly GWB-SKR254)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00254
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human Thrombospondin-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hThrombospondin-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Thrombospondin-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range1563-100000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
400X Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity1563 pg/mL
SpecificityThe Human Thrombospondin-1 ELISA is capable of recognizing both recombinant and naturally produced Human Thrombospondin-1 proteins. The antigens listed below were tested at 1000 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: CD36, Latent TGF-β1, Thrombospondin-2, Thrombospondin-4
Publications

Quantitative Comparative Proteomics Reveals Candidate Biomarkers for the Early Prediction of Gestational Diabetes Mellitus: A Preliminary Study. In Vivo. 34, 517-525 (2020). 32111749

Assay InfoQuantitative Colorimentric Sandwich ELISA
Description
Gene SymbolTHBS1
Gene Full Namethrombospondin 1
Alias Symbolsglycoprotein G;THBS;THBS-1;thrombospondin-1;thrombospondin-1p180;thrombospondin-p50;TSP;TSP1;TSP-1.
NCBI Gene Id7057
Protein NameP07996
Description of TargetThrombospondin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P07996
Protein Accession #NP_003237.2
Nucleotide Accession #NM_003246.3
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