Catalog No: OKAG00254 (Formerly GWB-SKR254)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00254
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Thrombospondin-1 ELISA Kit (Human) : 96 Wells (OKAG00254)
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||
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Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||
ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||
ELISA Kit Duration | 4.5 hours | ||||||||||||||||||
ELISA Kit Principle | The Aviva Human Thrombospondin-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hThrombospondin-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Thrombospondin-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||
ELISA Kit Range | 1563-100000 pg/ml | ||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||
Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||
Sensitivity | 1563 pg/mL | ||||||||||||||||||
Specificity | The Human Thrombospondin-1 ELISA is capable of recognizing both recombinant and naturally produced Human Thrombospondin-1 proteins. The antigens listed below were tested at 1000 ng/ml and did not exhibit significant cross reactivity or interference. • Human: CD36, Latent TGF-β1, Thrombospondin-2, Thrombospondin-4 | ||||||||||||||||||
Publications | Quantitative Comparative Proteomics Reveals Candidate Biomarkers for the Early Prediction of Gestational Diabetes Mellitus: A Preliminary Study. In Vivo. 34, 517-525 (2020). 32111749 | ||||||||||||||||||
Assay Info | Quantitative Colorimentric Sandwich ELISA | ||||||||||||||||||
Description |
Gene Symbol | THBS1 |
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Gene Full Name | thrombospondin 1 |
Alias Symbols | glycoprotein G;THBS;THBS-1;thrombospondin-1;thrombospondin-1p180;thrombospondin-p50;TSP;TSP1;TSP-1. |
NCBI Gene Id | 7057 |
Protein Name | P07996 |
Description of Target | Thrombospondin-1 |
Uniprot ID | https://www.uniprot.org/uniprot/P07996 |
Protein Accession # | NP_003237.2 |
Nucleotide Accession # | NM_003246.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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