Catalog No: OKCD02926
Size:96WELLS
Price: $725.00
SKU
OKCD02926
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Datasheets/ManualsPrintable datasheet for THBS1 ELISA Kit (Bovine) (OKCD02926)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)95-103%97-105%87-101%85-103%
EDTA Plasma (n=5)92-101%93-101%84-94%96-105%
Heparin Plasma (n=5)83-95%78-103%93-101%79-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 1 (THBS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombospondin 1 (THBS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombospondin 1 (THBS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thrombospondin 1 (THBS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)95-10299
EDTA Plasma (n=5)94-10197
Heparin Plasma(n=5)85-9388
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombospondin 1 (THBS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombospondin 1 (THBS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-THBS1 Microplate96 Wells (12 x 8 Well strips)
THBS1 Lyophilized Standard2
100X Biotinylated THBS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum and plasma
Sensitivity30 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thrombospondin 1 (THBS1). No significant cross-reactivity or interference between Thrombospondin 1 (THBS1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolTHBS1
Gene Full Namethrombospondin 1
Alias Symbolsglycoprotein G;THBS;thrombospondin-1.
NCBI Gene Id281530
Protein NameThrombospondin-1
Description of TargetAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties (By similarity). May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).
Uniprot IDQ28178
Protein Accession #NP_776621.1
Nucleotide Accession #NM_174196.1
Protein Size (# AA)1170
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