Catalog No: OKEH01728
Size:96T
Price: $600.00
SKU
OKEH01728
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology THAP4 ELISA Kit (Human) (OKEH01728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for THAP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for THAP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample THAP4 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.5% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
THAP4 Microplate96 Wells (12 x 8 Well strips)
THAP4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated THAP4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human THAP domain-containing protein 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTHAP4
Gene Full NameTHAP domain containing 4
Alias SymbolsCGI-36;epididymis secretory sperm binding protein;Nb(III);nitrobindin;peroxynitrite isomerase THAP4;PP238;THAP domain-containing protein 4.
NCBI Gene Id51078
Protein NameQ8WY91
Description of TargetTHAP domain-containing protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WY91
Protein Accession #NP_001157828.1
Nucleotide Accession #NM_001164356.1
Protein Size (# AA)577
Molecular Weight62 kDa
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