Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD00845 (Formerly GWB-KBBDR8)
Size:96WELLS
Price: $650.00
SKU
OKCD00845
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for TGM3 ELISA Kit (Mouse) (OKCD00845)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-99%89-97%94-101%82-94%
EDTA plasma(n=5)99-105%95-103%88-104%91-101%
heparin plasma(n=5)86-99%91-104%87-95%81-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 3, Epidermal (TGM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 3, Epidermal (TGM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 3, Epidermal (TGM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 3, Epidermal (TGM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-9383
EDTA plasma(n=5)83-9888
heparin plasma(n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 3, Epidermal (TGM3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 3, Epidermal (TGM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Tgm3 Microplate96 Wells (12 x 8 Well Strips)
Tgm3 Lyophilized Standard2 x 20 ng
100X Biotinylated Tgm3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.138 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transglutaminase 3, Epidermal (TGM3). No significant cross-reactivity or interference between Transglutaminase 3, Epidermal (TGM3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTgm3
Gene Full Nametransglutaminase 3, E polypeptide
Alias SymbolsAI893889;E polypeptide;protein-glutamine gamma-glutamyltransferase E;protein-glutamine-gamma-glutamyltransferase;TG E;TG(E);TGase E;TGase-3;TGE;transglutaminase E;transglutaminase-3;we.
NCBI Gene Id21818
Protein NameProtein-glutamine gamma-glutamyltransferase E
Description of TargetCatalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
Uniprot IDQ08189
Protein Accession #NP_033400
Nucleotide Accession #NM_009374.3
Protein Size (# AA)693
Write Your Own Review
You're reviewing:TGM3 ELISA Kit (Mouse) (OKCD00845)
Your Rating
We found other products you might like!