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Catalog No: OKEH01258 (Formerly GWB-R2SD9X)
Size:96T
Price: $525.00
SKU
OKEH01258
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGM2 ELISA Kit (Human) (OKEH01258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGM2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGM2 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.0% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
TGM2 Microplate96 Wells (12 x 8 Well strips)
TGM2 Lyophilized Standard2
100X Biotinylated TGM2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Human Protein-glutamine gamma-glutamyltransferase 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTGM2
Gene Full Nametransglutaminase 2
Alias SymbolsC polypeptide, protein-glutamine-gamma-glutamyltransferase;erythrocyte transglutaminase;G(h);heart G alpha(h);hhG alpha(h);hTG2;isopeptidase TGM2;protein G alpha(h);protein-glutamine deamidase TGM2;protein-glutamine dopaminyltransferase TGM2;protein-glutamine gamma-glutamyltransferase 2;protein-glutamine histaminyltransferase TGM2;protein-glutamine noradrenalinyltransferase TGM2;protein-glutamine serotonyltransferase TGM2;TG(C);TGase C;TGase H;TGase II;TGase-2;TGC;tissue transglutaminase;transglutaminase C;transglutaminase H;transglutaminase II;Transglutaminase-2;tTG;tTgase.
NCBI Gene Id7052
Protein NameP21980
Description of TargetProtein-glutamine gamma-glutamyltransferase 2
Uniprot IDhttps://www.uniprot.org/uniprot/P21980
Protein Accession #NP_004604.2
Nucleotide Accession #NM_004613.2
Protein Size (# AA)687
Molecular Weight77 kDa
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