Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02032 (Formerly GWB-V17PMP)
Size:96T
Price: $600.00
SKU
OKEH02032
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

TGFBR2 ELISA Kit (Mouse) : 96 Wells (OKEH02032)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGFBR2 ELISA Kit (Mouse) (OKEH02032) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFBR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFBR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFBR2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
TGFBR2 Microplate96 Wells (12 x 8 Well strips)
TGFBR2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SKP2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse TGF-beta receptor type-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTgfbr2
Gene Full Nametransforming growth factor, beta receptor II
Alias Symbols1110020H15Rik;AU042018;DNIIR;RIIDN;Tbet;Tbeta;TbetaRII;TbetaR-II;TBR;TBR-II;TGF-beta receptor II;TGF-beta receptor type II;TGF-beta receptor type-2;TGF-beta type II receptor;TGFR-2;transforming growth factor-beta receptor type II.
NCBI Gene Id21813
Protein NameQ62312
Description of TargetTGF-beta receptor type-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q62312
Protein Accession #NP_033397.3
Nucleotide Accession #NM_009371.3
Protein Size (# AA)592
Molecular Weight67 kDa
Write Your Own Review
You're reviewing:TGFBR2 ELISA Kit (Mouse) : 96 Wells (OKEH02032)
Your Rating
We found other products you might like!