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Catalog No: OKEH02925
Size:96T
Price: $600.00
SKU
OKEH02925
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TGFB3 ELISA Kit (Human) (OKEH02925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFB3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFB3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFB3 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
TGFB3 Microplate96 Wells (12 x 8 Well strips)
TGFB3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TGFB3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Transforming growth factor beta-3
Publications

Macrophage Subpopulation Dynamics Shift following Intravenous Infusion of Mesenchymal Stromal Cells. Mol Ther. 28, 2007-2022 (2020)

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolTGFB3
Gene Full Nametransforming growth factor beta 3
Alias SymbolsARVD;ARVD1;LDS5;prepro-transforming growth factor beta-3;RNHF;TGF-beta3;transforming growth factor beta-3 proprotein.
NCBI Gene Id7043
Protein NameP10600
Description of TargetTransforming growth factor beta-3
Uniprot IDhttps://www.uniprot.org/uniprot/P10600
Protein Accession #NP_003230.1
Nucleotide Accession #NM_003239.3
Protein Size (# AA)412
Molecular Weight47 kDa
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