TGFB1 ELISA Kit (Pig) (OKEH03770)

Catalog No: OKEH03770

Size:96T

Pig TGFB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TGFB1 ELISA Kit (Pig) (OKEH03770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFB1 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Component:
  • Anti-TGFB1 Microplate: 96 Wells (12 x 8 Well strips)
  • TGFB1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated TGFB1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 4.2 pg/mL
• Specificity: Natural and recombinant Pig Transforming growth factor beta-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TGFB1
• Gene Full Name: transforming growth factor beta 1
• Alias Symbols: TGF-BETA-1;transforming growth factor beta-1;transforming growth factor beta-1 proprotein;transforming growth factor, beta 1 (Camurati-Engelmann disease).
• NCBI Gene Id: 397078
• Protein Name: P07200
• Description of Target: Transforming growth factor beta-1
• Uniprot ID: https://www.uniprot.org/uniprot/P07200
• Protein Accession #: NP_999180.1
• Nucleotide Accession #: NM_214015.1
• Protein Size (# AA): 390
• Molecular Weight: 44 kDa