TFF1 ELISA Kit (Rat) (OKCD02902)

Catalog No: OKCD02902

Size:96WELLS

Rat TFF1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-90%	80-89%	94-105%	83-92%
  EDTA Plasma (n=5)	92-99%	95-103%	94-105%	90-98%
  Heparin Plasma (n=5)	92-105%	91-99%	99-105%	82-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 1 (TFF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trefoil Factor 1 (TFF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 1 (TFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Trefoil Factor 1 (TFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-88	82
  EDTA Plasma (n=5)	89-103	94
  Heparin Plasma(n=5)	89-103	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TFF1 Microplate: 96 Wells (12 x 8 Well strips)
  • TFF1 Lyophilized Standard: 2
  • 100X Biotinylated TFF1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 27 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1). No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cyba
• Gene Full Name: cytochrome b-245 alpha chain
• Alias Symbols: alpha-subunit p22;cytochrome b(558) alpha chain;cytochrome b-245 light chain;cytochrome b-245, alpha polypeptide;cytochrome b558 alpha-subunit;cytochrome b558 subunit alpha;neutrophil cytochrome b 22 kDa polypeptide;p22 phagocyte B-cytochrome;p22phox;p22-phox;Phox;superoxide-generating NADPH oxidase light chain subunit.
• NCBI Gene Id: 79129
• Protein Name: Cytochrome b-245 light chain
• Description of Target: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
• Uniprot ID: Q62737
• Protein Accession #: NP_001002992.1
• Nucleotide Accession #: NM_001002992.1
• Protein Size (# AA): 81