Catalog No: OKEH02920
Size:96T
Price: $525.00
SKU
OKEH02920
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology TFF1 ELISA Kit (Human) (OKEH02920) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TFF1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TFF1 and incubated. The TFF1 found in the sample or standards competes with the biotinylated TFF1 for limited binding sites on the immobilized anti-human TFF1 antibody. Excess unbound biotinylated TFF1 and sample or standard TFF1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TFF1 captured in the well and inversely proportional to the amount of TFF1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.9% (n = 20) Mean Inter-assay CV%: < 7.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.098 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Trefoil factor 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | TFF1 |
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Gene Full Name | trefoil factor 1 |
Alias Symbols | BCEI;breast cancer estrogen-inducible protein;breast cancer estrogen-inducible sequence;D21S21;gastrointestinal trefoil protein pS2;HP1.A;HPS2;pNR-2;polypeptide P1.A;protein pS2;pS2;trefoil factor 1. |
NCBI Gene Id | 7031 |
Protein Name | P04155 |
Description of Target | Trefoil factor 1 |
Uniprot ID | https://www.uniprot.org/uniprot/P04155 |
Protein Accession # | NP_003216.1 |
Nucleotide Accession # | NM_003225.2 |
Protein Size (# AA) | 84 |
Molecular Weight | 9 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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