TFEB Antibody (OAAF02178)

Catalog No: OAAF02178 (Formerly GWB-ASD434)

Size:100 ug

Western blot analysis of extracts from mouse lung/mouse muscle cells, using TFEB Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TFEB Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human TFEB.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Concentration: 1 mg/ml
• Specificity: TFEB antibody detects endogenous levels of total TFEB protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1,000
  IHC: 1:50~100
  ELISA: 1:20,000
  Optimal dilutions should be determined by the end user.
• Reference: Carr C.S., Mol. Cell. Biol. 10:4384-4388(1990).
  Kuiper R.P., Nucleic Acids Res. 32:2315-2322(2004).
  Mungall A.J., Nature 425:805-811(2003).

Target Info

• Gene Symbol: TFEB
• Gene Full Name: transcription factor EB
• Alias Symbols: ALPHATFEB;BHLHE35;class E basic helix-loop-helix protein 35;T-cell transcription factor EB;TCFEB;transcription factor EB.
• NCBI Gene Id: 7942
• Protein Name: Transcription factor EB
• Description of Target: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
• Uniprot ID: P19484
• Protein Accession #: NP_001161299.2
• Molecular Weight: 52 kDa