TFAP2A Antibody (OAAF01732)

Catalog No: OAAF01732 (Formerly GWB-ASD007)

Size:100 ug

Western blot analysis of extracts from COLO205 cells, using AP-2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using AP-2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human AP-2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: AP-2 antibody detects endogenous levels of total AP-2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:10000
• Reference: ShunJiang Yu, J. Biol. Chem., May 2003; 278: 19597 - 19602.
  TT Chen, Mol. Cell. Biol., Jun 1997; 17: 3056 - 3064.
  Oscar B. Goodman, J. Biol. Chem., Oct 1995; 270: 23768.
  Didier Jean, J. Biol. Chem., Jun 1998; 273: 16501
• Publications:

  Li, J. et al. A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells. FEBS Lett. 584, 3198-202 (2010). 20547158

Target Info

• Gene Symbol: TFAP2A|TFAP2B
• Gene Full Name: transcription factor AP-2 alpha|transcription factor AP-2 beta
• Alias Symbols: activating enhancer binding protein 2 beta;activating enhancer-binding protein 2-alpha;Activating enhancer-binding protein 2-beta;activator protein 2;AP-2;AP-2 transcription factor;AP-2alpha;AP2-B;AP-2B;AP2-beta;AP-2beta;AP2TF;BOFS;PDA2;TFAP2;transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha);transcription factor AP-2-alpha;transcription factor AP-2-beta.
• NCBI Gene Id: 7020|7021
• Protein Name: Transcription factor AP-2-alpha|Transcription factor AP-2-beta
• Description of Target: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.|Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
• Uniprot ID: P05549|Q92481
• Protein Accession #: NP_001027451.1
• Molecular Weight: 48 kDa