TFAM ELISA Kit (Rat) (OKCA01941)

Catalog No: OKCA01941

Size:96 Wells

Rat TFAM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology Tfam ELISA Kit (Rat) (OKCA01941) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tfam has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tfam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Tfam captured in the well.
• ELISA Kit Range: 23.5 - 1,500 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	101	99-103
  EDTA Plasma (n=4)	90	84-95

• ELISA Kit Component:
  • Tfam Microplate: 96 Wells (12 x 8 Well strips)
  • Tfam Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated Tfam Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates And Cell Lysates
• Sensitivity: 5.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: TFAM
• Gene Full Name: transcription factor A, mitochondrial
• Alias Symbols: Mttfa
• NCBI Gene Id: 83474
• Protein Name: Transcription factor A, mitochondrial
• Description of Target: Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.
• Uniprot ID: Q91ZW1
• Protein Accession #: NP_112616.1
• Nucleotide Accession #: NM_031326.1