Catalog No: OKEH02147
Size:96T
Price: $525.00
SKU
OKEH02147
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TBXAS1 ELISA Kit (Human) (OKEH02147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBXAS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBXAS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBXAS1 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 10.6% (n = 20)
ELISA Kit Component
ComponentAmount
TBXAS1 Microplate96 Wells (12 x 8 Well strips)
TBXAS1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TBXAS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Thromboxane-A synthase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTBXAS1
Gene Full Namethromboxane A synthase 1
Alias SymbolsBDPLT14;CYP5;CYP5A1;cytochrome P450 5A1;cytochrome P450, family 5, subfamily A, polypeptide 1;GHOSAL;hydroperoxy icosatetraenoate dehydratase;platelet, cytochrome P450, subfamily V;THAS;thromboxane A synthase 1 (platelet);thromboxane-A synthase;TS;TXA synthase;TXAS;TXS.
NCBI Gene Id6916
Protein NameP24557
Description of TargetThromboxane-A synthase
Uniprot IDhttps://www.uniprot.org/uniprot/P24557
Protein Accession #NP_001052.2
Nucleotide Accession #NM_001061.4
Protein Size (# AA)533
Molecular Weight61 kDa
Write Your Own Review
You're reviewing:TBXAS1 ELISA Kit (Human) (OKEH02147)
Your Rating
We found other products you might like!