Catalog No: OKCD01546 (Formerly GWB-KBBKW1)
Size:96WELLS
Price: $650.00
SKU
OKCD01546
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Datasheets/ManualsPrintable datasheet for Tat ELISA Kit (Rat) (OKCD01546)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Sodium Citrate Plasma (n=5)80-96%81-88%84-98%87-101%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antibody specific to thrombin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATIII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Sodium Citrate Plasma (n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombin/Antithrombin Complex (TAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombin/Antithrombin Complex (TAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Tat Microplate96 Wells (12 x 8 Well Strips)
Tat Lyophilized Standard2 x 20 ng
100X Biotinylated Tat Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thrombin/Antithrombin Complex (TAT). No significant cross-reactivity or interference between Thrombin/Antithrombin Complex (TAT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSerpinc1
Gene Full Nameserpin family C member 1
Alias Symbolsantithrombin III;antithrombin-III;Coagulation factor II;serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1;serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1;serpin peptidase inhibitor, clade C (antithrombin), member 1.
NCBI Gene Id304917
Protein NameProthrombin|Serine (Or cysteine) peptidase inhibitor, clade C (Antithrombin), member 1
Description of TargetThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Uniprot IDP18292
Protein Accession #NP_036800.1
Nucleotide Accession #NM_012668.2
Protein Size (# AA)454
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