TAP1 ELISA Kit (Human) (OKCD00893)

Catalog No: OKCD00893 (Formerly GWB-KBBCL6)

Size:96WELLS

Human TAP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Antigen Peptide Transporter 1 (TAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Antigen Peptide Transporter 1 (TAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Antigen Peptide Transporter 1 (TAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Antigen Peptide Transporter 1 (TAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TAP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TAP1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TAP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.134 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Antigen Peptide Transporter 1 (TAP1). No significant cross-reactivity or interference between Antigen Peptide Transporter 1 (TAP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TAP1
• Gene Full Name: transporter 1, ATP binding cassette subfamily B member
• Alias Symbols: ABC transporter, MHC 1;ABC17;ABCB2;antigen peptide transporter 1;APT1;ATP-binding cassette sub-family B member 2;ATP-binding cassette, sub-family B (MDR/TAP), member 2;D6S114E;peptide supply factor 1;peptide transporter involved in antigen processing 1;peptide transporter PSF1;peptide transporter TAP1;PSF1;PSF-1;really interesting new gene 4 protein;RING4;TAP1*0102N;TAP1N;transporter 1 ATP-binding cassette sub-family B;transporter 1, ATP-binding cassette, sub-family B (MDR/TAP);transporter associated with antigen processing;transporter, ATP-binding cassette, major histocompatibility complex, 1.
• NCBI Gene Id: 6890
• Protein Name: Antigen peptide transporter 1
• Description of Target: Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
• Uniprot ID: Q03518
• Protein Accession #: NP_000584
• Nucleotide Accession #: NM_000593.5
• Protein Size (# AA): 808