Catalog No: OKCD00349 (Formerly GWB-KBBHO9)
Size:96WELLS
Price: $675.00
SKU
OKCD00349
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Datasheets/ManualsPrintable datasheet for TAGLN ELISA Kit (Human) (OKCD00349)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-95%78-91%97-104%82-98%
EDTA plasma(n=5)87-95%81-96%85-101%80-88%
heparin plasma(n=5)80-90%97-105%79-94%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transgelin (TAGLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transgelin (TAGLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transgelin (TAGLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transgelin (TAGLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)96-104101
EDTA plasma(n=5)90-9794
heparin plasma(n=5)79-9689
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transgelin (TAGLN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transgelin (TAGLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TAGLN Microplate96 Wells (12 x 8 Well Strips)
TAGLN Lyophilized Standard2 x 10 ng
100X Biotinylated TAGLN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transgelin (TAGLN). No significant cross-reactivity or interference between Transgelin (TAGLN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTAGLN
Gene Full Nametransgelin
Alias Symbols22 kDa actin-binding protein;epididymis secretory sperm binding protein;Protein WS3-10;SM22;SM22-alpha;SMCC;smooth muscle protein 22-alpha;TAGLN1;transgelin;transgelin variant 2;WS3-10.
NCBI Gene Id6876
Protein NameTransgelin
Description of TargetActin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Uniprot IDQ01995
Protein Accession #NP_001001522
Nucleotide Accession #NM_001001522.1
Protein Size (# AA)201
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